Anti-Neuroligin-4 Antibody

Mouse Monoclonal Antibody specific to Neuroligin-4

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Neuroligin-4, NL-4

Gene Name

Nlgn4l

NCBI Gene ID

UniProt

B0F2B4

Cellular Locus

Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic cell membrane. Note=Detected at glycinergic postsynapses in retina. Detected on dendritic spines on cultured neurons.

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 782-945 (intracellular C-terminus) of mouse Neuroligin-4.

Target Antigen

Neuroligin 4-like

Target

Neuroligin-4

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Cell surface protein involved in cell-cell-interactions. Plays a role in the formation or maintenance of synaptic junctions via its interactions (via the extracellular domains) with neurexin family members. Plays a role in synaptic signal transmission. {PubMed:18434543, PubMed:21282647}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~125-130kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse Neuroligin-4. It does not cross-react with other Neuroligins.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

Neuroligin-4 is a neuronal cell surface protein and a member of the type-B carboxylesterase- lipase protein family. Members of this family may act as splice site-specific ligands for beta- neurexins and may be involved in the formation and remodeling of central nervous system synapses. Neuroligin-4 interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants.