NSE gamma antibody
NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE ; -phospho-D-glycerate hydrolyase
UniProt
P09104
Reactivity
Human
Immunogen
A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-451M
Conjugation
Unconjugated
Field of Research
Metabolism Research
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative. Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa
Available Sizes
Frequently Asked Questions
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