Cytokeratin 15 antibody
Cytokeratin 15 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
CK15; Cytokeratin 15; K1CO; Ka15; Keratin 15 basic; Keratin 15 beta; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15
UniProt
P19012
Reactivity
Human
Immunogen
Recombinant full-length human KRT15 protein
Clonality
Monoclonal
Clone
MSVA-615M
Conjugation
Unconjugated
Dilution
1:50-1:100
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: All cells of the surface squamous epithelium and most squamous cells of the crypts should show strong KRT15 staining. Negative Control: Tonsil: All lymphocytes and blood vessels must not show any KRT15 staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa
Available Sizes
Frequently Asked Questions
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