P16 antibody
P16 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
CDK4 inhibitor p16 INK4; CDK4I; CDKN2A; Cell cycle negative regulator beta; CMM2; Cyclin dependent kinase 4 inhibitor A; Melanoma p16 inhibits CDK4; MLM; MTS1; Multiple tumor suppressor 1; p14; p16; p19; P19ARF; TP16
UniProt
P42771
Reactivity
Human
Immunogen
Recombinant full-length human p16INK4a protein
Clonality
Monoclonal
Clone
MSVA-016R
Conjugation
Unconjugated
Field of Research
Cancer Biology
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Pancreas: at least a moderate staining is expected in islets of Langerhans Negative Control: Pancreas: staining should be absent in acinar cells, Normal cervix uteri: p16 staining should be absent in epithelial cells (in some cases, few cells may show weak staining) Cellular Localization: Nuclear and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG
Available Sizes
Frequently Asked Questions
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