AIF1 antibody
AIF1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Specifications
Product Name Alternative
Allograft inflammatory factor 1 (AIF1) ; balloon angioplasty responsive transcription (BART1) ; IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)
UniProt
P55008
Reactivity
Human
Immunogen
Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-955M
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages. Negative Control: Colon: AIF1 immunostaining should be absent in all non-inflammatory cells. Cellular Localization: Cytoplasmic and cell surface. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2c, kappa
Available Sizes
Frequently Asked Questions
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