Swine anti Rabbit Ig light chains kappa and lambda (free and bound)

As tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rabbit serum and purified IgG fractions and light chain preparations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab’)2 subunits, and with free light chains of both types; also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. This antiserum is not kappa or lambda chain specific. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As precipitating antiserum in a variety of immunodiffusion techniques including immunoelectrophoresis and double and single radial immunodiffusion Mancini, Ouchterlony). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Precipitation assays. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single immunodiffusion, use 1% antiserum in agar gel to establish a standard curve.

Delipidated, heat inactivated lyophilized stable whole serum; No preservative added, as it may interfere with the antibody activity; No foreign protein added; Total protein and IgG concentration in the antiserum are comparable to those of pooled swine serum; No foreign proteins added; It is reconstituted by adding 1 mL sterile distilled water

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the product