Goat anti-tdTomato Polyclonal antibody

Goat polyclonal antibody to tdTomato (red fluorescent protein) conjugated to DyLight® 680. tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Product Name Alternative

Cherry fluorescent protein; DsRed, mCherry, red fluorescent protein, RFP antibody.

UNSPSC Description

Red Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Reacts against tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.

Immunogen

Purified recombinant peptide produced in E. coli

Target Antigen

Purified recombinant peptide produced in E. coli

Immunogen Type

Recombinant protein

Target

Anti-tdTomato, DyLight®680

Clonality

Polyclonal

Isotype

IgG

Conjugation

DyLight®680

Type

Primary

Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK

Applications

WB, IF, IHC-F

Purification

Epitope affinity purified

Concentration

2.5 mg/mL

Dilution

WB:1:500-1:2,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Form

Polyclonal antibody supplied as a 100 µl (2.5 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.