CD3 antibody

CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

UniProt

P07766

Reactivity

Human

Immunogen

A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)

Clonality

Monoclonal

Clone

MSVA-003R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells) . Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining. Cellular Localization: Cell surface. Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.