Caspase 3/7 and DAPI Double Staining Kit

The Caspase 3/7 Substrates (Green) are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD), which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. DAPI co-staining of cells with Caspase 3/7 Substrates allows simultaneous differentiation of late-apoptotic cells, necrotic cells, and caspase 3/7 enzyme activity during different apoptotic periods.