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Phospho-Nucleolin (T84) Recombinant Rabbit Monoclonal Antibody [JE64-18]

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'- TTAGGG-3' repeats.

Product Specifications

CAS Number

9000-83-3

Product Name Alternative

C23 FLJ45706 MS1116 NCL Nucl NUCL_HUMAN Nucleolin Protein C23

Abbreviation

Nucleolin, Protein C23, NCL

Swiss Prot

P19338 Human

Cellular Locus

Cytoplasm, Nucleus, nucleolus

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic phosphopeptide corresponding to residues around T84 of human Nucleolin.

Isotype

IgG

Clone

JE64-18

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

293T cell lysate, K-562 cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 77 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes

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