Phospho-Nucleolin (T84) Recombinant Rabbit Monoclonal Antibody [JE64-18]
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'- TTAGGG-3' repeats.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
C23 FLJ45706 MS1116 NCL Nucl NUCL_HUMAN Nucleolin Protein C23
Abbreviation
Nucleolin, Protein C23, NCL
Swiss Prot
P19338 Human
Cellular Locus
Cytoplasm, Nucleus, nucleolus
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic phosphopeptide corresponding to residues around T84 of human Nucleolin.
Isotype
IgG
Clone
JE64-18
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P
Positive Control
293T cell lysate, K-562 cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, human tonsil tissue.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IHC-P: 1:200
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 77 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
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