Anti-Human IgG H&L Chain Cross-Adsorbed Antibody, Alkaline Phosphatase conjugate
Goat Polyclonal Antibody specific to Human IgG, H&L Chain, Alk Phos Conj
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-Human IgG, H&L Chain, Alk Phos Conj Goat Polyclonal Antibody
Host
Goat
Reactivity
Human
Immunogen
Human IgG
Target
Human IgG H&L Chain Cross-Adsorbed
Clonality
Polyclonal
Isotype
Whole IgG
Type
Antibody
Applications
WB, IHC, ICC, ELISA
Field of Research
Secondary Antibodies
Purification Method
Purified by peptide immuno-affinity chromatography
Concentration
0.5 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
HEPES
Additionnal Information
ELISA: 1:500-1:5,000
<br><br>Western Blot: 1:200-1:1,000
<br><br>Immunohistochemistry: 1:200-1:500
<br><br>Immunocytochemistry: 1:200-1:500
<br><br>These are recommended dilutions only.
<br><br>End user should determine optimal dilutions for their applications.
Storage Conditions
Store at 2-8°C. Expires 1 year from date of receipt.
Specificity
Antiserum was cross-adsorbed on bovine, chicken, horse, mouse, pig, rabbit, and rat immunosorbents to remove cross-reactive antibodies. By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. Reactivity with non-immunoglobulin serum proteins is not detected.
Formulation
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA and 0.09% NaN3
Buffer pH
pH 7.1
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