Thermolysin from Geobacillus stearothermophilus

<strong>Thermolysin from Geobacillus stearothermophilus</strong>_x000D_ <strong>Catalog number:</strong> B2014172_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 5 mg_x000D_ <strong>Molecular Weight or Concentration:</strong> 30-350 units/mg protein_x000D_ <strong>Supplied as:</strong> Powder_x000D_ <strong>Applications:</strong> molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -20°C_x000D_ <strong>Keywords:</strong> Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Ke Q, Chen A, Minoda M, Yoshida H. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus Food Chem Toxicol. 2013 Sep;59:541-8._x000D_ 2: Ripp S, Turunen P, Minot ED, Rowan AE, Blank KG. Deciphering Design Principles of Frster Resonance Energy Transfer-Based Protease Substrates: Thermolysin-Like Protease from Geobacillus stearothermophilus as a Test Case ACS Omega. 2018 Apr 12;3(4):4148-4156._x000D_ 3: Amelunxen RE, Murdock AL. Mechanisms of thermophily CRC Crit Rev Microbiol. 1978;6(4):343-93._x000D_ 4: Naitow H, Matsuura Y, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Tanaka R, Tanaka T, Sugahara M, Kobayashi J, Nango E, Iwata S, Kunishima N. Protein-ligand complex structure from serial femtosecond crystallography using soaked thermolysin microcrystals and comparison with structures from synchrotron radiation Acta Crystallogr D Struct Biol. 2017 Aug 1;73(Pt 8):702-709._x000D_ 5: D_rrschmidt P, Mansfeld J, Ulbrich-Hofmann R. Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate Biophys Chem. 2010 Mar;147(1-2):66-73._x000D_ 6: Eijsink VG, Matthews BW, Vriend G. The role of calcium ions in the stability and instability of a thermolysin-like protease Protein Sci. 2011 Aug;20(8):1346-55._x000D_ 7: Mansfeld J, Petermann E, D_rrschmidt P, Ulbrich-Hofmann R. The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus Protein Expr Purif. 2005 Feb;39(2):219-28._x000D_ 8: Van den Burg B, Vriend G, Veltman OR, Venema G, Eijsink VG. Engineering an enzyme to resist boiling Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2056-60._x000D_ 9: Mansfeld J, Ulbrich-Hofmann R. The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents Biotechnol Bioeng. 2007 Jul 1;97(4):672-9._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/26619013">10: Sierra RG, Gati C, Laksmono H, Dao EH, Gul S, Fuller F, Kern J, Chatterjee R, Ibrahim M, Brewster AS, Young ID, Michels-Clark T, Aquila A, Liang M, Hunter MS, Koglin JE, Boutet S, Junco EA, Hayes B, Bogan MJ, Hampton CY, Puglisi EV, Sauter NK, Stan CA, Zouni A, Yano J, Yachandra VK, Soltis SM, Puglisi JD, DeMirci H. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II Nat Methods. 2016 Jan;13(1):59-62. </a>_x000D_ _x000D_ <strong>Products Related to Thermolysin from Geobacillus stearothermophilus can be found at</strong> <a href="https://moleculardepot.com/product-category/Enzymes/"> Enzymes</a>