DiD (DiIC18 (5) )

The molecular formula of DiD (DiIC18 (5) ) is C67H103ClN2O3S, the molecular weight is 1052.1, and the CAS number is 362596-00-7. DiD dye is a member of the lipophilic fluorescent dye family, which can be used to stain cell membranes and other lipid-soluble biological structures. When DiD is bound to the cell membrane, its fluorescence intensity is greatly enhanced, and these dyes have a high quenching constant and excited state lifetime. Once the cell is stained, such dyes diffuse throughout the cell membrane and at optimal concentrations stain the entire cell membrane. DiD (far red fluorescence) can be used to image live cells and flow analysis. DiD can be excited by 633 nm He-Ne laser and has longer excitation wavelength and emission wavelength than DiI (a common cell fluorescent dye), which is more valuable in cell and tissue staining. After DiD staining, the fixation of paraformaldehyde (no other reagents such as methanol) can be carried out, but the process of permeabilization after staining is not recommended. In addition, plasma membrane staining was also well performed after fixed permeabilization with 0.1% TritonX-100 at room temperature. Based on the calculation of using 100 μL of staining working solution at a concentration of 5 μMeach time, 10 mg of working solution can be used about 19,009 times.

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