CheKine™ Micro Glutamate Synthase (GOGAT) Assay Kit

CheKine™ Micro Glutamate Synthase (GOGAT) Assay Kit provides a simple, convenient and rapid GOGAT activity detection method, which is suitable for the detection of animal and plant tissue, cell, bacteria, serum, plasma and other samples. The detection principle is that GOGAT uses NADH as the electron donor to catalyze the amino transfer of glutamine to α-ketoglutaric acid to form two-molecule glutamate. The decreasing rate of NADH absorbance in 340 nm can reflect the GOGAT activity.

Product Specifications

Background

Glutamate synthase (GOGAT) mainly exists in prokaryotes, yeasts and non-green tissues of higher plants. Together with glutamine synthase (GS), glutamate synthase (GS) forms the GS/GOGAT cycle and participates in the regulation of ammonia assimilation.

Tag

GOGAT

Type

Cell analysis

Components

• ReagentI•ReagentII•ReagentIII

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

• Determination of GOGAT activity in a variety of biological samples such as Animal and Plant Tissue, Cell, Bacteria, Serum, Plasma. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.

Applications Notes

The kit provides a simple method for detecting Glutamate Synthase (GOGAT) activity in a variety of biological samples such as serum, plasma, tissues, cells , plants and bacteria.

Recommended Usage

• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.