CheKine™ Micro Aconitase (ACO) Activity Assay Kit

Aconitase, an enzyme in the tricarboxylic acid cycle, catalyzes the conversion of citric acid to isocitrate. Citric acid itself is not easy to be oxidized. Under the action of aconitase, the hydroxyl group is transferred from the β carbon atom to the α carbon atom through the reaction of dehydration and water addition to generate isocitric acid that is easy to deoxidize, for further oxidative decarboxylation reaction be prepared.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Determination of Aconitase (ACO) Activity in a variety of biological samples such as serum, plasma, tissues, cells and plants. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.