CheKine™ Micro Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Assay Kit

1, 5-ribulose bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) is a key enzyme in plant photosynthesis, which not only controls the fixation of CO2, but also controls the distribution of carbon to the Calvin cycle and the photorespiration cycle. Its activity directly affects the photosynthetic rate.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

CheKine™ Micro Ribulose bisphosphate carboxylase/oxygenase (Rubisco) Assay Kit provides a simple method for detecting Rubisco activity in plant tissues. One molecule of ribulose-1,5-diphosphate (RuBP) combines with one molecule of CO2 to produce two molecules of 3-phosphoglyceric acid (PGA) . Glyceraldehyde-3-phosphate can be produced by PGA through the action of additional 3-phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase, which is accompanied by NADH oxidation to produce NAD+. NADH has a characteristic absorption peak at 340 nm, while NAD+ does not. Rubisco activity is calculated by measuring the rate of decrease in light absorption at 340 nm.