CheKine™ Micro 6-Phosphofructokinase (PFK) Activity Assay Kit

Phosphofructokinase (PFK) is widely found in animals, plants, microorganisms and cultured cells. It is responsible for the conversion of fructosae 6-phosphate and ATP into fructosae 1,6 diphosphate and ADP. It is one of the key regulatory enzymes in the glycolysis process. Phosphofructokinase (PFK) activity in bacterial cell supernatant and other samples

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Detect PFK activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells • Detailed sample preparation and results calculation methods are provided

CheKine™ Micro 6-Phosphofructokinase (PFK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity

• Do not mix or substitute reagents or materials from other kit lots or vendors. • It is advisable to measure serum potassium as soon as possible after blood collection. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.