CheKine™ Micro Reducing Sugar (RS) Assay Kit

Reduction sugars are widely in animals, plants, microorganisms and cultured cells. The reduction sugars in plants mainly include glucose, fructose and maltose.Glucose and fructose are not only the main substrates of respiration, but also the monosaccharide of sucrose, starch and cellulose.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• simple, sensitive, rapid Reducing Sugar detection method. • compatible to animal or plant tissues, bacteria, cell cultures (adherent or suspension) . • Provides detailed sample preparation and results calculation methods.

CheKine™ Micro Reduction Sugar (RS) Assay kit provides a simple, convenient, rapid and colorimetric reducing sugar detection method. This kit can detect the reduction sugars concentration from liquid samples, such as animal and plant tissues homogenate, bacteria, cells and serum (plasma) . The principle of kit is that 3,5-dinitrosalicylic acid can be reduced to brown-red amino compounds by reducing sugar in alkaline solution. There is a characteristic absorption peak 540nm. In a certain concentration range, the reducing sugar content is linearly related to the absorbance of 540nm. According to the standard curve, the reducing sugar content in the sample can be calculated.

• Do not mix the components between different batch numbers and manufacturers, otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the appropriate temperature before to use. • In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.If the sample value is higher than the maximum standard value, please further dilute the sample with distilled water. Pay attention to multiply by the dilution factor when calculating the result. • DNS reagent is toxic. Please take protective measures when operating.