CheKine™ Micro Blood Glucose Assay Kit

Blood glucose is the main form of sugar transport in mammalian body. Blood glucose concentration is regulated by nervous system and hormones. Hyperglycemia and hypoglycemia occur when the regulation is out of balance. Diabetes, increased intracranial pressure and dehydration can cause hyperglycemia, and after meals, mental stress can cause physiological hyperglycemia. Patients with pancreatic β-cell hyperplasia or tumors, pituitary gland, adrenal cortex and hypothyroidism, and severe liver disease all cause hypoglycemia. In addition, hunger and strenuous exercise can cause temporary hypoglycemia. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of blood glucose can be very important in both disease research and drug discovery processes.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

CheKine™ Micro Blood Glucose Assay Kit provides a simple, convenient, rapid and colorimetric blood glucose detection method.The principle of kit is glucose oxidase can catalyze the oxidation of glucose to produce gluconic acid and hydrogen peroxide.peroxidase catalyzes hydrogen peroxide oxidation of 4-aminoantipyrine coupled phenol produce colored compounds which with a characteristic absorption peak at 505 nm. Within a certain concentration range, the glucose concentration has a linear relationship with the absorbance at 505 nm. According to the standard curve, the glucose concentration in the sample can be calculated.

• Do not mix the components between different batch numbers and manufacturers, otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.If the sample value is higher than the maximum standard value, please further dilute the sample with distilled water. Pay attention to multiply by the dilution factor when calculating the result. • Reagent I and Reagent II has certain toxicity. Please take protective measures when operating.