CheKine™ Micro Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Assay Kit

Mitochondrial Isocitrate Dehydrogenase (ICDHM) is widely present in mitochondria of animals, plants, microorganisms, and cultured cells. It can catalyze Isocitrate decarboxylic reaction and produce α-Ketoglutarate, and reduce NADP+ to NADPH.

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• simple, convenient, rapid and colorimetric Mitochondrial Isocitrate Dehydrogenase (ICDHm) activity detection method • compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi. • Provides detailed sample preparation and results calculation methods.

CheKine™ Micro Mitochondrial Isocitrate Dehydrogenase (ICDHm) Assay Kit provides a simple, convenient, rapid and colorimetric ICDHm activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi. The principle is that ICDHM can catalyze NADP + reduction to produce NADPH, NADPH has an absorption peak at 340 nm, and ICDHM activity can be calculated by the change of light absorption at 340 nm.

• ΔA of Test Tube should be between 0.01-0.3, the sample should be diluted further if ΔA of Test Tube > 0.3. • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • All samples and reagents should be on ice to avoid denaturation and deactivation . • Don't recommend to test too many samples in one time , because enzyme activity is calculated by the variation of the absorbance value per unit time . • Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight , the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction. • Please to extract ICDHm from fresh samples in order to ensure the enzyme activity . • Please reduce the tissues weight if the OD values is too high . Or reduce the volume of Extraction Buffer if the OD values is too low .