CheKine™ Micro Pyruvate Dehydrogenase (PDH) Activity Assay Kit

Pyruvate dehydrogenase (PDH) is a rate-limiting enzyme while pyruvate dehydrogenase complex (PDHC) catalyzes pyruvic acid oxidation and decarboxylation. After decarboxylationand, produce hydroxyethyl-TPP, which connects glycolysis with the tricarboxylic acid cycle.

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• simple, convenient, rapid and colorimetric PDH activity detection method. • compatible to various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension) . • Provides detailed sample preparation and results calculation methods.

CheKine™ Micro Pyruvate Dehydrogenase (PDH) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PDH activity detection method, which is compatible to various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension) . The detection principle is that PDH can catalyze pyruvate dehydrogenation, and make 2,6-Dichlorophenolindophenol (2,6-DCPIP) reduced, the changes of 2,6-dichlorohenol could be detected by reading the absorption at 605 nm. And the PDH activity could be obtained by calculating the reduction rate of 2,6-Dichlorophenolindophenol.

• ΔA of Test Tube should be between 0.01-0.3, the sample should be diluted further if ΔA of Test Tube > 0.3. • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • All samples and reagents should be on ice to avoid denaturation and deactivation . • Don't recommend to test too many samples in one time, because enzyme activity is calculated by the variation of the absorbance value per unit time. • Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight, the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction. • Please to extract PDH from fresh samples in order to ensure the enzyme activity. • Please take protective measures when operating, because Reagent II and Reagent IV has certain toxicity.