CheKine™ Micro Citrate Synthase (CS) Activity Assay Kit

Citrate Synthase (CS) widely exist in the mitochondrial matrix of animals, plants, microorganisms, and cultured cells, and is first enzyme of the Kreb's cycle, and is one of the main regulatory sites of Kreb's cycle.

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• simple, sensitive, rapid and colorimetric CS activity detection method. • compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) . • Provides detailed sample preparation and results calculation methods.

CheKine™ Micro Citrate Synthase (CS) Activity Assay Kit provides a simple, sensitive, rapid and colorimetric CS activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) . CS activity is determined depending on that CS can catalyze acetyl CoA and oxamic acid to produce citamoyl co-enzyme A, further hydrolysis to produce citric acid; this reaction causes colorless DTNB to transform into yellow TNB, TNB has a characteristic absorbing value at 412 nm.The activity of citrate synthase could be obtained by calculated the increase rate of the light absorption at 412nm.

• ΔA of Test Tube should be between 0.01-0.3, the sample should be diluted further if ΔA of Test Tube > 0.3. • Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • All samples and reagents should be on ice to avoid denaturation and deactivation. • Don't recommend to test too many samples in one time , because enzyme activity is calculated by the variation of the absorbance value per unit time. • Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight , the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction. • Please to extract CS from fresh samples in order to ensure the enzyme activity. • Please take protective measures when operating, because ReagentII has certain toxicity.