CheKine™ Micro Mitochondrial complex IV Activity Assay Kit

Mitochondrial respiratory chain complex IV, also known as cytochrome C oxidase, is also a common component of the main and branch pathways of the mitochondrial respiratory chain. It is responsible for catalyzing the oxidation of reduced cytochrome C, and ultimately transferring electrons to oxygen to produce water.

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• Detect the enzyme activity of complex IV in plant tissues, animal tissues, cells and other samples. • Provides detailed sample preparation and results calculation methods.

Abbkine CheKine™ Micro Mitochondrial complex IV Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Reduced cytochrome C has characteristic light absorption at 550nm. Mitochondrial respiratory chain complex IV could catalyze reduced cytochrome C to oxidized cytochrome C. Therefore, the decreasing rate of light absorption at 550nm can reflect the enzyme activity of mitochondrial respiratory chain complex IV.

• Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components.• Change pipette tips frequently to avoid cross contamination between components.• Ensure that all components and equipment are at the proper temperature before starting the experiment.• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.The samples should be diluted with ReagentIIand then measured again If the absorbance values is too high (> 1), or ∆A > 0.2. Pay attention to multiply by the dilution factor when calculating the result. While the ΔA value is too small, the sensitivity can be improved by increasing the sample volume added. • The protein concentration of the sample needs to be determined by users.