CheKine™ Micro Mitochondrial complex III Activity Assay Kit

Mitochondrial respiratory chain complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely present in the mitochondria of animals, plants, microorganisms and cultured cells. It plays a key role at the main and branch pathways in the mitochondrial respiratory electron transport chain. Complex III could transfer the hydrogen of the reduced CoQ to cytochrome C , and produce reduced cytochrome C.

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• Detect the enzyme activity of complex III in plant tissues, animal tissues, and cell samples. • Provides detailed sample preparation and results calculation methods.

Abbkine CheKine™ Micro Mitochondrial Complex III Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex III could transfer the hydrogen from reduced CoQ to cytochrome C to produce reduced cytochrome C. Unlike oxidized cytochrome C, reduced cytochrome C has characteristic light absorption at 550 nm. So, the increasing of light absorption rate can reflect the activity of Complex III enzymes, and Abbkine CheKine™ Micro Mitochondrial complex III Activity Assay Kit can detect animal and plant tissues and cell samples.

• Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.• Avoid bubbles while mixing or redissolving components.• Change pipette tips frequently to avoid cross contamination between components.• Ensure that all components and equipment are at the proper temperature before starting the experiment.• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. If the absorbance values is too high (above 1), the samples should be diluted with ReagentIIand then measured again . Pay attention to multiply by the dilution factor when calculating the result. • The protein concentration of the sample needs to be determined by users.•Note that Reagent III has certain toxicity, Please take protective measures during operation.