CheKine™ Micro Mitochondrial complex I Activity Assay Kit

Mitochondrial respiratory chain complex I (EC 1.6.5. 3), also known as NADH-CoQ reductase or NADH dehydrogenase , is the largest protein complex in the mitochondrial intima. Complex I is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, and at the same time it can reduce O2 to produce O2-, which is the main part of the respiration electron transport chain to produce O2-. The activity of Complex I can not only reflect the status of responsibility electron transfer chain (ETC), but also reflect the production of reactive oxygen species (ROS) .

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• Detect the enzyme activity of complex I in plant tissues, animal tissues, cells. • Provides detailed sample preparation and results calculation methods

Abbkine CheKine™ Micro Mitochondrial Complex I Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340 nm to calculate the activity of Complex I .

• Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.• Avoid bubbles while mixing or redissolving components.•Change pipette tips frequently to avoid cross contamination between components.• Ensure that all components and equipment are at the proper temperature before starting the experiment.• In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. If the absorbance values is too high (above 1.5) or ΔA is greater than 0.4, the samples should be dilute with ReagentIIand then measured again . Pay attention to multiply by the dilution factor when calculating the result. If ΔA is too small, the sensitivity can be improved by increasing the sample volume added.•The protein concentration of the sample needs to be determined by users.