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CheKine™ Micro Cysteine (Cys) Assay Kit

Abbkine CheKine™ Cysteine (Cys) Colorimetric Assay Kit is designed for detecting the Cys conten in the sample.

Product Specifications

Background

Protein contains three sulfur-containing amino acids: methionine, cystine and Cys. Among them, Cys is the only sulfur-containing amino acid containing a sulfhydryl group, which is converted from methionine and can be mutually converted with cystine. Cys participates in the formation of protein disulfide bonds and is often a component of the active center of proteins. It can also provide sulfhydryl groups for other physiological and biochemical reactions.

Product Name Alternative

(+) -2-Amino-3-mercaptopropionic acid; 2-Amino-3-mercaptopropanoic acid; C; Cys; CySH; Cysteine; L-beta-mercaptoalanine; L-Cysteine; L-CYSTEINE FREE BASE CRYSTALLINE; (S) - (-) -Cysteine; L-Cysteine Base

Tag

Cys

Type

Cell analysis

Limit Of Detection

0.156 μmol/mL

Components

• Extraction Buffer • Assay Buffer • Chromogen • Cys Substrate

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

• Determination of Cys conten in a variety of biological samples such as Liquid sample, plant tissues, cells, and bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.

Applications Notes

The kit provides a simple method for detecting the Cys conten in a variety of biological samples such as Liquid sample, tissues, cells, and bacteria. Cys reduces phosphotungstic acid to produce tungsten blue, which has an absorption peak at 600 nm; the Cys content is calculated by the absorbance at 600 nm.

Other Product Names

(+) -2-Amino-3-mercaptopropionic acid; 2-Amino-3-mercaptopropanoic acid; C; Cys; CySH; Cysteine; L-beta-mercaptoalanine; L-Cysteine; L-CYSTEINE FREE BASE CRYSTALLINE; (S) - (-) -Cysteine; L-Cysteine Base

Recommended Usage

• Do not mix or substitute reagents or materials from other kit lots or vendors. • Avoid foaming or bubbles when mixing or reconstituting components. • Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.

Calibration Range

0.156-10 μmol/mL

Available Sizes

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