CheKine™ Micro Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit

Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) is widely present in animals, plants, microorganisms and cells. It is an enzyme that catalyzes the reaction of phosphoenol acetic acid with gas phase to produce oxaloacetic acid and presents an irreversible reaction. The operation of the acid cycle plays an important regulatory role.

Phosphopyruvate carboxylase; Phosphoenolpyruvic carboxylase; PHOSPHOENOLPYRUVATE CARBOXYLASE; Phosphoenolpyruvic acid cocarboxylase; Phospho (enol) pyruvate Carboxylase corn; Carboxylase, phosphopyruvate (phosphate)

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Determination of Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, plasma, bacterial. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

The kit provides a simple method for detecting Phosphoenolpyruvate Carboxylase (PEPC) (EC 4.1.1.31) activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, plasma, bacterial. In the assay, Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate and CO2 to produce oxaloacetate and HPO42-, and malate dehydrogenase further catalyzes oxaloacetate and NADH to produce malate and NAD+. The rate of NADH decrease at 340 nm can reflect Phosphoenolpyruvate carboxylase (PEPC) activity.

Phosphopyruvate carboxylase; Phosphoenolpyruvic carboxylase; PHOSPHOENOLPYRUVATE CARBOXYLASE; Phosphoenolpyruvic acid cocarboxylase; Phospho (enol) pyruvate Carboxylase corn; Carboxylase, phosphopyruvate (phosphate)

• Do not mix or substitute reagents or materials from other kit lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.•Avoid foaming or bubbles when mixing or reconstituting components.• To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.•Ensure all reagents and solutions are at the appropriate temperature before starting the assay.