CheKine™ Micro Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Aspartate aminotransferase (AST/GOT) is widely present in animals, plants, microorganisms and cultured cells. It catalyzes the reversible amino reaction and is an important enzyme for amino acid metabolism. Serum AST activity level is an important biochemical indicator of liver damage, which can reflect the severity of liver damage. In addition, AST has the highest content in cardiomyocytes and is generally used as an auxiliary examination for myocardial infarction and myocarditis in clinical practice.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Determination of AST/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. • Standard and standard curve line are provided.

The kit provides a simple method for detecting AST/GOT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, Aspartate aminotransferase (AST) catalyzes the transamination reaction of α-ketoglutarate and L-aspartic acid at 37°C and pH 7.4 to produce glutamic acid and pyruvate; Pyruvate can interact with 2,4- Dinitrophenylhydrazine to produce pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect AST/GOT activity.

• Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.