CheKine™ Micro Total Cholesterol (TC) Assay Kit

Total cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Determination of Total Cholesterol (TC) content in a variety of biological samples such as Serum, Plasma, Animal Tissues, Cells, Bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA) . Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.

• Do not mix or substitute reagents or materials from other kit lots or vendors. • There are volatile substances in the kit, gloves and masks should be worn during the experiment, and the reagent bottle cap should be tightly closed in time after opening. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.