CheKine™ Micro NAD+ kinase (NADK) Activity Assay Kit

Abbkine CheKine™ Micro NAD+ kinase (NADK) Activity Assay Kit is designed for detecting NADK activity in the sample.

Product Specifications

Background

NADK (EC 2.7.1.23) is widely found in animals, plants, microorganisms and cultured cells. It is the only enzyme that can catalyze the phosphorylation of NAD+ to NADP+ in organisms. It can catalyze the phosphorylation reaction of NAD (H) with ATP or inorganic polyphosphate [poly (P) ] as a phosphoryl donor to generate NADP (H) . Therefore, NAD kinase plays an important role in the synthesis of NADP (H) and the regulation of the balance between NAD (H) and NADP (H) .

Tag

NADK

Type

Cell analysis

Components

• Extraction Buffer • Assay Buffer I • Assay Buffer II• Substrate Mix• Enzyme Mix

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

• Determination of NADK activity in a variety of biological samples such as Serum, Plasma, Animal and Plant Tissues, Cells, Bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Storage at -20°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.

Applications Notes

The kit provides a simple method for detecting NADK activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, NADK catalyzes the phosphorylation of NAD+ to produce NADP+; NADP+ can be reduced to NADPH by glucose-6-phosphate dehydrogenase. NADPH has a characteristic absorption peak at 340 nm. The rate of NADPH increase at 340 nm can reflect NADK activity.

Recommended Usage

• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Substrate Mix and Enzyme Mix must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25°C (general species) or 37°C (mammal) .