Terminal Deoxynucleotidyl Transferase (TdT)

Terminal Deoxynucleotidyl Transferase (TdT), expressed in

Product Specifications

Background

Terminal deoxynucleotidyl transferase (TdT), also known as terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells. Generally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases, it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends.

Product Name Alternative

DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase

Gene ID

X04122

Accession Number

P06526

Tag

TdT

Type

Cytokines and proteins

Applications

No tag

Purity

> 90 % as determined by SDS-PAGE

Activity

Testing in progress

Molecular Weight

58.3 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stored at -20°C.

Protein Length

The recombinant bovine TdT consists of 520 amino acids and migrates with an apparent molecular mass of 58.3 kDa as estimated in SDS-PAGE under reducing conditions.

Formulation

Liquid in sterile 50 mM K2HPO4, 100 mM NaCl, 1 mM DTT, 0.1% Tween20, 1% BSA, 50% Glycerol, pH 6.5.

Other Product Names

TdT; DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase

Recommended Usage

The Equilibration Buffer (5x) stock solution is stored at low temperature, resulting in a small amount of component precipitation. Please invert and mix before use. And Equilibration Buffer (5x) contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately