One-step TUNEL Apoptosis Assay Kit (Red Fluorescence)

One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.

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• Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. • Signal can be easily detected (Ex/Em = 555nm/565 nm) .

Stable for at least 12 months at -20°C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components.

Abbkine One-step TUNEL Apoptosis Assay Kit (Orange Fluorescence) relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected (Ex/Em = 555nm/565 nm) .

The reaction buffer contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately. Do not drink, eat or smoke when using.