Senescence β-Galactosidase Staining Kit

Cells are the basic unit of biological structure and function, as well as the basic unit of biological aging. Cellular senescence is morphologically manifested as degeneration of cell structure, such as nuclear membrane depression, which eventually leads to nuclear membrane collapse, chromatin structure changes, and the number of hyperdiploid and abnormal polyploid cells increases; cell membrane fragility increases. Sexual permeability decreases, and the types and numbers of membrane receptors and the sensitivity to ligands change; lipofuscin accumulates in cells, and many organelles and intracellular structures undergo degeneration. Cell senescence is physiologically manifested as functional decline and low metabolism, such as cell cycle arrest, loss of cell replication ability, weakened responsiveness to mitogenic stimulation, and changes in responsiveness to pro-apoptotic factors; intracellular enzyme active centers are oxidized , Enzyme activity decreased, protein synthesis decreased, etc. The senescent cells can no longer replicate, but they still maintain metabolically active and stain positive for senescence-associated β-galactosidase activity, which is considered to be a biomarker of cellular senescence.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Simple and optimized procedure. • Fast and convenient. • Suitable for aging detection of cultured cells and tissue sections.• Cell senescence β-galactosidase staining kit only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.

Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.

Senescence β-Galactosidase Staining Kit provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated β-galactosidase (SA-β-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-β-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells.

• Fixative solutions are toxic and corrosive to humans, use special care when operating, and take care to protect effectively.• X-gal solutions stored at -20°C or 4°C will thaw completely by freezing at room temperature or in a 37°C water bath for 2-5 min with appropriate shaking.• Cellular senescence the β- galactosidase staining reaction is dependent on specific pH conditions and cannot be performed in a carbon dioxide incubator. A higher concentration of carbon dioxide in the incubator can affect the pH of the staining working solution, which leads to staining failure. For multi well plates, it may be sealed with parafilm or plastic wrap to prevent evaporation.• If there is a pellet after reagent thaw or before use, ensure that the pellet has dissolved in its entirety before use. Reagent C when just removed from the kit, there may be a small precipitate on the bottom of the tube, which is normal, the precipitate dissolves completely after thorough mixing, and it is necessary to ensure that it is used after all has dissolved. A small flocculent precipitate may also be present when formulating the staining working solution, dissolve completely after shaking to mix, and this must be ensured to be used only after all has dissolved.