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Human IL-3 Protein, His tag (Animal-Free)

Human IL-3 protein, His tag (Animal-Free), expressed in HEK 293 Cells

Product Specifications

Background

Human interleukin-3 (hIL-3) is a therapeutically important cytokine involved in the maturation and differentiation of various cells of the immune system. IL-3 predominantly produced by activated T lymphocytes, stimulates both endothelial cell proliferation and functional activation. Unlike other cytokines, IL-3 is a cytokine with a multilineage potential and broad spectrum of target cells and it plays a vital role in hematopoiesis. Due to its common receptor subunit, the action of IL-3 shows functional redundancy with other cytokines such as the granulocyte-macrophage colony-stimulating factor and IL-5. IL-3 has been successfully used in ameliorating radiation-induced bone marrow aplasia and similar conditions.

Product Name Alternative

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple) ; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor

Gene ID

3562

Accession Number

P08700

Tag

IL-3

Type

Cytokines and proteins

Applications

His tag

Purity

> 95 % as determined by SDS-PAGE

Activity

Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 6.87ng/mL.

Molecular Weight

16 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: < 1 EU per µg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized Human IL-3 protein, His tag (Animal-Free) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant human IL-3 protein consists of 140 amino acids and predicts a molecular mass of 16 kD.The apparent molecular mass of the human IL-3 is approximately 17-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile PBS, pH 7.4.

Applications Notes

Human

Other Product Names

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple) ; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human IL-3 protein in the buffer which we provide not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

Preparation

HEK 293 Cells

Accession Number URL

https://www.uniprot.org/uniprot/P08700

AA Sequence

Amino acid sequence derived from human IL-3 protein (NP_000579.2, Met1-Phe152) was expressed with a polyhistidine tag at the C-terminus.

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