Rat TNF-α Protein
Rat TNF-α protein, expressed in E. coli
Product Specifications
Background
Tumor necrosis factor α (TNF-α), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-α. TNF-α protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-α is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Product Name Alternative
TNF; TNFA; TNFSF2; DIF ; Tumor Necrosis Factor ; Tumor Necrosis Factor Ligand Superfamily Member; Tumor Necrosis Factor (TNF Superfamily, Member 2) ; Tumor Necrosis Factor Ligand 1F ; Tumor Necrosis Factor-Alpha ; TNF Superfamily, Member 2 ; TNLG1F 3; TNF 5
Gene ID
24835
Accession Number
P16599
Tag
TNF-α
Type
Cytokines and proteins
Applications
No tag
Purity
> 98 % as determined by SDS-PAGE
Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells. The ED50 for this effect is typically 1.208 pg/mL. The specific activity of recombinant Rat TNF-α is approximately >8.3 x 105 IU/mg
Molecular Weight
17.2 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 0.1 EU per µg of the protein as determined by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Rat TNF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Rat TNF-α consists of 156 amino acids and has a predicted molecular mass of 17.2 kDa.
Formulation
Lyophilized from sterile 20 mM tris 50 mM NaCl, pH 8.0.
Applications Notes
Rat
Other Product Names
TNF; TNFA; TNFSF2; DIF ; Tumor Necrosis Factor ; Tumor Necrosis Factor Ligand Superfamily Member; Tumor Necrosis Factor (TNF Superfamily, Member 2) ; Tumor Necrosis Factor Ligand 1F ; Tumor Necrosis Factor-Alpha ; TNF Superfamily, Member 2 ; TNLG1F 3; TNF 5
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat TNF-alpha protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprot/P16599
AA Sequence
Amino acid sequence derived from Rat TNF-α (P16599) (Leu80-Leu235) was expressed.
Available Sizes
Curated Selection
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