What is the catalog number for Rat bFGF Protein?

The catalog number for Rat bFGF Protein is PRP1210.

What sizes are available for Rat bFGF Protein?

Rat bFGF Protein is available in the following size(s): 10 µg, 50 µg, 100 µg.

How many pack sizes does Rat bFGF Protein come in?

Rat bFGF Protein is available in 3 different pack sizes.

Is Rat bFGF Protein in stock?

Rat bFGF Protein is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

Rat bFGF Protein

Rat bFGF protein, expressed in E. coli

Product Specifications

Background

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic over-expression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.

Product Name Alternative

Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

Gene ID

54250

Accession Number

P13109

Tag

BFGF

Type

Cytokines and proteins

Applications

No tag

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.0015 ng/mL.The specific activity of recombinant Rat bFGF approximately >6.67 x 108 IU/mg

Molecular Weight

16.2 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized Rat bFGF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant Rat bFGF protein consists of 145 amino acids (N-Met) and has a predicted molecular mass of 16.2 KD. The apparent molecular mass of the Rat FGF2 is approximately 16.2 KD in SDS-PAGE under reducing conditions due to glycosylation.

Formulation

Lyophilized from sterile 20 mM Tris,150 mM NaCl, pH8.0.

Applications Notes

Rat

Other Product Names

Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat bFGF protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.