Human GM-CSF Protein

Human GM-CSF protein, expressed in E. coli

Product Specifications

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML) .

Product Name Alternative

GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2

Gene ID

1437

Accession Number

P04141

Tag

GM-CSF

Type

Cytokines and proteins

Applications

No tag

Purity

> 98 % as determined by SDS-PAGE

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemia cells. The ED50 for this effect is 0.05-0.5 ng/mL.

Molecular Weight

14 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized Human GM-CSF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Protein Length

The recombinant human GM-CSF consists of 128 amino acids and has a predicted molecular mass of 14 kDa.

Formulation

Lyophilized from sterile PBS, pH 7.4.

Applications Notes

Human

Other Product Names

GM-CSF; GMCSF; Granulocyte-macrophage colony-stimulating factor; CSF2

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human GM-CSF protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.