Anti-Osteocalcin Antibody

Mouse Monoclonal Antibody specific to Bovine Osteocalcin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Bone Gla protein, BGP, γ-carboxyglutamic acid-containing protein

Gene Name

BGLAP

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=BGLAP">BGLAP</a>

UniProt

P02818

Cellular Locus

Secreted.

Host

Mouse

Reactivity

Human, Rat, Rabbit, Sheep, Goat, Bovine, Porcine

Immunogen

Bovine osteocalcin

Target Antigen

Osteocalcin

Target

Osteocalcin

Clonality

Monoclonal

Isotype

IgG3

Type

Antibody

Applications

ELISA, IHC

Field of Research

Hormones/Proteins

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration) then dilute further for applications in PBS or media that is identical to that used in the assay system.

Format

Purified

Form

Lyophilized

Buffer

Tris

Function

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Additionnal Information

Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120. Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions. Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Endusers should determine optimal concentrations for their applications.

Storage Conditions

Store stock solution (2. 0 mg/ml) in aliquots at -20 for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.

Specificity

Epitope within residues 21-31; human, bovine, rat, rabbit, pig, goat, and sheep.

Formulation

20 mM TBS, pH7.5, containing 1.0% bovine serum albumin, lyophilized

Target Background

Osteocalcin is a noncollagenous, 49 amino acid glutamate-rich polypeptide bone matrix protein with a molecular weight of about 5800Da. Osteoblasts produce osteocalcin and incorporate it into the bone matrix. Osteocalcin is released into the circulation from the matrix during bone resorption and, therefore, is considered a marker of bone turnover rather than a specific marker of bone formation.