Mouse anti FITC (Fluorescein isothiocyanate)
Product Specifications
Background
The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversion of fluorescein-labeled detection into immunoenzymatic assays.
CAS Number
9007-83-4
Host
Mouse
Isotype
IgG1
Clone
F4/1
Conjugation
FITC
Type
Monoclonal Antibody
Source
F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.
Applications
WB, ELISA, Flow Cytometry
Field of Research
Ancillary Reagents
Purification Method
Protein A/G Chromatography
Assay Principle
This antibody can be used for Western blotting (1-2 µg/mL), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 µg/mL) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Balogh P; et al. 'Hapten mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody.' J. Immunol. Methods 1994, 169, 35-40.
Shipping Conditions
Ambient Temperature, freeze upon arrival
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
WB
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