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HRP, Donkey Anti-Sheep IgG

HRP conjugated Donkey Anti-Sheep IgG (H+L) secondary antibody

Product Specifications

Background

Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

CAS Number

9007-83-4

Reactivity

Sheep

Immunogen

Sheep IgG whole molecule

Clonality

Polyclonal

Conjugation

HRP

Tag

Donkey Anti-Sheep IgG

Type

Sample Preparation and detection

Applications

WB-IHC-P-ELISA

Purification Method

Affinity purified using solid phase Sheep IgG (H&L) with finally > 95% purity based on SDS-PAGE

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody reacts with whole molecule sheep IgG. It also reacts with heavy chains of sheep IgG, and light chains of all other sheep immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

Formulation

Liquid solution

Buffer pH

Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:100000), IHC-P (1:500-1:2000), ELISA (1:5000-1:100000) .

Tested Applications

WB#IHC#IP#ELISA

Host or Source

Donkey

Isotype

Donkey IgG

Available Sizes

Curated Selection

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