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IPKine™ Anti-GFP Magnetic IP Kit

The kit contains high-quality Anti-GFP magnetic beads and the necessary reagents for immunoprecipitation that have been optimized and validated, making immunoprecipitation (IP) experiments simpler, more convenient and more efficient.

Product Specifications

Background

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

CAS Number

9007-83-4

Reactivity

Mammals, Bacteria

Immunogen

Synthetic Peptide

Conjugation

Magnetic Beads

Tag

GFP

Type

Sample Preparation and detection

Applications

IP

Components

Non-Denaturing Lysis BufferTBS (10X) Anti-GFP Magnetic BeadsMouse IgG Magnetic BeadsElution BufferNeutralization BufferSDS-PAGE Loading Buffer (5X)

Precautions

The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes.

Features & Benefits

1.Efficient binding capacity: ≥0.6 mg GFP Tag protein/mL Magnetic Beads.• 2.Two elution methods: acid elution and denatured elution.• 3.The kit contains high-quality Anti-GFP magnetic beads and all validated immunoprecipitation necessary reagents.• 4.Ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Store according to the recommended storage conditions of each component, stable for 12 months.

Applications Notes

Anti-Green Fluorescent Protein-Tag (GFP-Tag) Magnetic Beads are prepared by covalently coupling Anti-GFP-Tag Mouse Monoclonal Antibody to crosslinked Magnetic Beads. The Optimized Anti-GFP Magnetic Beads have more efficient antigen binding capacity. This kit provides two elution methods, including acid elution and denatured elution.

Other Product Names

Immunoprecipitation; KTI2024; Anti-GFP; GFP; Magnetic Beads; IP; IP Kit; GFP tag; Kit; Anti-GFP IP; Anti-GFP IP Kit; GFP epitope; IPKine™ Anti-GFP Magnetic IP Kit; GFP Kit; GFP IP Kit

Tested Applications

IP

Available Sizes

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