IPKine™ HRP, Mouse Anti-Rabbit IgG HCS
IPKine™ HRP conjugated Mouse Anti-Rabbit IgG heavy chain specific secondary antibody
Product Specifications
Background
Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.
CAS Number
9007-83-4
Reactivity
Rabbit
Immunogen
Rabbit IgG heavy chain
Clonality
Monoclonal
Conjugation
HRP
Tag
Mouse Anti-Rabbit IgG
Type
Sample Preparation and detection
Applications
WB-IP
Purification Method
Affinity purified using solid phase Rabbit IgG (H+L) light chain with finally > 95% purity based on SDS-PAGE.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination) . Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy) . Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots) .
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
The antibody reacts with kappa heavy chains on rabbit IgG, while it doesn’t react with the light chain of rabbit IgG. It's cross-reaction with human, mouse, rat, goat, sheep and bovine serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins.
Formulation
Liquid solution
Buffer pH
Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
Applications Notes
The suggested dilution ratio is: WB: 1:1,000-1:10,000 (1:2000 optimal), IP: 1:1,000-1:10,000 (1:2000 optimal) .
Tested Applications
WB#IP
Host or Source
Mouse
Isotype
Mouse IgG
Available Sizes
Frequently Asked Questions
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