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IPKine™ HRP, Mouse Anti-Rabbit IgG HCS

IPKine™ HRP conjugated Mouse Anti-Rabbit IgG heavy chain specific secondary antibody

Product Specifications

Background

Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.

CAS Number

9007-83-4

Reactivity

Rabbit

Immunogen

Rabbit IgG heavy chain

Clonality

Monoclonal

Conjugation

HRP

Tag

Mouse Anti-Rabbit IgG

Type

Sample Preparation and detection

Applications

WB-IP

Purification Method

Affinity purified using solid phase Rabbit IgG (H+L) light chain with finally > 95% purity based on SDS-PAGE.

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination) . Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy) . Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots) .

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody reacts with kappa heavy chains on rabbit IgG, while it doesn’t react with the light chain of rabbit IgG. It's cross-reaction with human, mouse, rat, goat, sheep and bovine serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins.

Formulation

Liquid solution

Buffer pH

Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.

Applications Notes

The suggested dilution ratio is: WB: 1:1,000-1:10,000 (1:2000 optimal), IP: 1:1,000-1:10,000 (1:2000 optimal) .

Tested Applications

WB#IP

Host or Source

Mouse

Isotype

Mouse IgG

Available Sizes

Frequently Asked Questions

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