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Anti-His Tag Rabbit Polyclonal Antibody

Featured Anti-His Tag Rabbit Polyclonal Antibody, specially designed for your taged protein quantification and localization.

Product Specifications

Background

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

CAS Number

9007-83-4

Product Name Alternative

His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag

Reactivity

Mammals, Bacteria

Immunogen

Synthetic Peptide

Clonality

Polyclonal

Tag

His

Type

Sample Preparation and detection

Applications

WB

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Formulation

Liquid solution

Buffer pH

Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000) .

Other Product Names

His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag

Tested Applications

WB#IF#IP

Host or Source

Rabbit

Isotype

Rabbit IgG

Available Sizes

Frequently Asked Questions

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