Anti-His Tag Mouse Monoclonal Antibody (5C3)
Featured Anti-His Tag Mouse Monoclonal Antibody (5C3), specially designed for your taged protein quantification and localization.
Product Specifications
Background
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
CAS Number
9007-83-4
Product Name Alternative
His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag
Reactivity
Mammals, Bacteria
Immunogen
Synthetic Peptide
Clonality
Monoclonal
Tag
His
Type
Sample Preparation and detection
Applications
WB-IF-IP
Purification Method
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Formulation
Liquid solution
Buffer pH
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1500), IP (1:300) .
Other Product Names
His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag
Tested Applications
WB#IF#IP
Host or Source
Mouse
Isotype
Mouse IgG
Available Sizes
Frequently Asked Questions
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