Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)

Featured Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), specially designed for your taged protein quantification and localization.

Product Specifications

Background

The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

CAS Number

9007-83-4

Product Name Alternative

DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope

Reactivity

Mammals, Bacteria

Immunogen

Synthetic Peptide

Clonality

Monoclonal

Tag

Flag

Type

Sample Preparation and detection

Applications

WB-IF-IP

Purification Method

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Concentration

1 mg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Formulation

Liquid solution

Buffer pH

Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400) .