Anti-β-Actin Mouse Monoclonal Antibody (1C7)
Featured Anti-β-Actin Mouse Monoclonal Antibody (1C7), specially designed for your immunoassay as internal control or location staining.
Product Specifications
Background
β-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. β-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa.
CAS Number
9007-83-4
Product Name Alternative
ACTB; Actin; cytoplasmic 1; Beta-actin
Gene ID
60
Accession Number
P60709
Reactivity
Human-Mouse-Rat-Monkey-Dog-Chicken-Rabbit-Insect-Hamster
Immunogen
Synthetic Peptide
Clonality
Monoclonal
Tag
ACTB
Type
Sample Preparation and detection
Applications
WB-IHC-P-IF
Purification Method
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Observed Molecular Weight
43
Formulation
Liquid solution
Buffer pH
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:100-1:400) .
Other Product Names
β Actin; ACTB; Actin; cytoplasmic 1; Beta-actin
Tested Applications
WB#IHC#IF#IP
Host or Source
Mouse
Accession Number URL
http://www.uniprot.org/uniprot/P60709
Isotype
Mouse IgG1
Available Sizes
Frequently Asked Questions
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