What is the catalog number for EliKine™ Mouse VEGF ELISA Kit?

The catalog number for EliKine™ Mouse VEGF ELISA Kit is KTE7016.

What sizes are available for EliKine™ Mouse VEGF ELISA Kit?

EliKine™ Mouse VEGF ELISA Kit is available in the following size(s): 48 Tests, 96 Tests.

How many pack sizes does EliKine™ Mouse VEGF ELISA Kit come in?

EliKine™ Mouse VEGF ELISA Kit is available in 2 different pack sizes.

Is EliKine™ Mouse VEGF ELISA Kit in stock?

EliKine™ Mouse VEGF ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

EliKine™ Mouse VEGF ELISA Kit

EliKine™ Mouse VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse VEGF-A.

Product Specifications

Background

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

CAS Number

7732-18-5

Product Name Alternative

VEGFA; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor

Gene ID

22339

Accession Number

Q00731

Reactivity

Mouse

Tag

VEGFA

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

8 pg/ml

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

EliKine™ Mouse VEGF ELISA Kit has high sensitivity and excellent specificity for detection of Mouse VEGF-A. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

EliKine™ Mouse VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF-A in samples. An antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

VEGFA; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.