What is the catalog number for EliKine™ Mouse IL-2 ELISA Kit?

The catalog number for EliKine™ Mouse IL-2 ELISA Kit is KTE7006.

What sizes are available for EliKine™ Mouse IL-2 ELISA Kit?

EliKine™ Mouse IL-2 ELISA Kit is available in the following size(s): 48 Tests, 96 Tests.

How many pack sizes does EliKine™ Mouse IL-2 ELISA Kit come in?

EliKine™ Mouse IL-2 ELISA Kit is available in 2 different pack sizes.

Is EliKine™ Mouse IL-2 ELISA Kit in stock?

EliKine™ Mouse IL-2 ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

EliKine™ Mouse IL-2 ELISA Kit

EliKine™ Mouse IL-2 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-2.

Product Specifications

Background

Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types. IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes .

CAS Number

7732-18-5

Product Name Alternative

IL 2; IL2; IL-2; TCGF; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion

Gene ID

16183

Accession Number

P04351

Reactivity

Mouse

Tag

IL2

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

100 pg/mL

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

EliKine™ Mouse IL-2 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-2. No significant cross-reactivity or interference between Mouse IL-2 and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

EliKine™ Mouse IL-2 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-2 in samples. An antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-2 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

IL 2; IL2; IL-2; TCGF; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.