What is the catalog number for EliKine™ Mouse IL-1α ELISA Kit?

The catalog number for EliKine™ Mouse IL-1α ELISA Kit is KTE7004.

What sizes are available for EliKine™ Mouse IL-1α ELISA Kit?

EliKine™ Mouse IL-1α ELISA Kit is available in the following size(s): 48 Tests, 96 Tests.

How many pack sizes does EliKine™ Mouse IL-1α ELISA Kit come in?

EliKine™ Mouse IL-1α ELISA Kit is available in 2 different pack sizes.

Is EliKine™ Mouse IL-1α ELISA Kit in stock?

EliKine™ Mouse IL-1α ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

EliKine™ Mouse IL-1α ELISA Kit

EliKine™ Mouse IL-1α ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-1α.

Product Specifications

Background

Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1α and IL-1β.These belong to a family of cytokines known as the interleukin-1 superfamily.Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells.

CAS Number

7732-18-5

Product Name Alternative

Hematopoietin 1; IL 1 alpha; Il 1a; IL1; IL1 ALPHA; IL1A; IL1F1; Ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha

Gene ID

16175

Accession Number

P01582

Reactivity

Mouse

Tag

IL1A

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

4 pg/mL

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

EliKine™ Mouse IL-1α ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-1α. No significant cross-reactivity or interference between Mouse IL-1α and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

EliKine™ Mouse IL-1α ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1α in samples. An antibody specific for IL-1α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1α bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

Hematopoietin 1; IL 1 alpha; Il 1a; IL1; IL1 ALPHA; IL1A; IL1F1; Ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.